10 essential tools for molecular biology students and researchers
Calculate amino acid composition and statistical analysis of protein sequences. Groups residues by chemical properties.
Compute molecular weight for DNA sequences as single- or double-stranded, linear or circular molecules. Supports IUPAC degenerate bases.
Calculate residue counts, dinucleotide frequencies, GC content, and grouped totals (purines vs pyrimidines) for DNA sequences.
Calculate the molecular weight of protein sequences including fusion tags (FLAG, HA, His6, GST, MBP) and extinction coefficient at 280nm.
Analyze PCR primer properties: melting temperature (3 methods), GC content, self-annealing, hairpin formation, and PCR suitability.
Generate standard curves and quantify unknown samples from qPCR data. Calculates R², PCR efficiency, and back-calculates concentrations.
Calculate nucleic acid concentration from OD260 measurements using Beer-Lambert law. Supports ssDNA, dsDNA, and ssRNA.
Convert between mass and moles for ssRNA and dsRNA sequences. Calculates molecular weight, copy number, and moles of ends.
Convert between mass and moles for ssDNA and dsDNA sequences. Uses exact nucleotide weights when sequence is provided.
Calculate dilution volumes using the C₁V₁=C₂V₂ formula. Instantly find how much stock solution and solvent you need.