Generate standard curves and quantify unknown samples from qPCR data
| Sample Name | Dilution Factor | Cq 1 | Cq 2 | Cq 3 | Avg Cq |
|---|
1. Enter known concentrations and Ct values for your standards (min 3 points, ideally 5+)
2. Add unknown samples with their Cq values and dilution factors
3. Click "Generate Standard Curve" — the tool fits a linear regression to log₁₀(conc) vs Ct
4. R² ≥ 0.98 and efficiency 90–110% indicate a reliable standard curve